| NE-DNA

NE is a serine protease belonging to the same family as chymotrypsin, exhibiting broad substrate specificity. Neutrophil elastase is secreted by neutrophils during inflammatory processes, degrading both bacteria and host tissues. It also localizes to neutrophil extracellular traps (NETs) via high affinity for DNA, an unusual characteristic for serine proteases. Like other serine proteases, it contains a charge relay system comprising a catalytic triplet of histidine, aspartic acid, and serine residues. These residues are dispersed throughout the primary sequence of the polypeptide but converge in the folded three-dimensional conformation of the protein. The gene encoding neutrophil elastase, ELA2, consists of five exons.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human NE-DNA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
 

| Linearity

Samples were spiked with high concentrations of Human NE-DNA and diluted with Universal Diluent to produce samples with values within the range of the assay.